# Time Constant

This section covers the cell anology of an electrical circuit with resistor and capacitor components.

An electrical equivalent of a cell with three ion channels can be represented as follows:

Where:

- C
_{m}is the membrane capacitance formed by the lipid bilayers - G
_{k+}is the Potassium conductance (1/Resistance) - E
_{k+}is the reversal potential of Potassium (from the Equilibrium Table) -97 mV) - G
_{Na+}is the Sodium conductance - E
_{Na+}is the reversal potential of Sodium +67 mV - G
_{Ci+}is the Chloride conductance - E
_{Ci+}is the reversal potential of Chloride -85 mV

**Using Ohm's law:**

\begin{array}{l}V=IR\\ I=VG\end{array}

Where:

- V=Voltage across the ion channel
- I=Ionic current through the channel
- R=Resistance to the flow
- G=The conductance

Since R is 1/conductance we can rewrite the equation to be

I=VG

**Capacitance:**

\begin{array}{l}Q=CV\\ I=C\frac{dV}{dT}\end{array}

where:

- Q = the charge in the capacitor
- C = the capacitance
- V=The voltage across the capacitor

We know that the current (I) is equal to the rate of change of the charge. Differentiated to give the capacitive current flow.

Therefor the total trans-membrane current (**I _{m}**)consists of four current flows.

\begin{array}{l}{I}_{m}={I}_{c}+{I}_{{k}^{+}}+{I}_{N{a}^{+}}+{I}_{C{I}^{-}}\\ {I}_{c}={C}_{m}\frac{d{V}_{m}}{dt}\\ {I}_{{k}^{+}}=({V}_{m}-{E}_{{k}^{+}}){G}_{{k}^{+}}\\ {I}_{N{a}^{+}}=({V}_{m}-{E}_{N{a}^{+}}){G}_{N{a}^{+}}\\ {I}_{C{I}^{-}}=({V}_{m}-{E}_{C{I}^{-}}){G}_{C{I}^{-}}\end{array}

So to change the membrane potential we have to change the ionic conductances for Potassium, Sodium or Chloride.

For example:
What happens to V_{m} if Potassium channel (K^{+}) Opens.

The Potassium ions will escape the inside of the cell membrane and move down their concentration gradient to the outside leaving a negative charge inside the cell. This causes the membrane potential to move toward the Potassium reversal potential (about -90 mV).

The complete voltage across the capacitor can be modeled with the RC time constant equation:

## {V}_{m}={E}_{k}(1-{e}^{-t/RC)})

The term RC is the membrane time constant. If the cell has a high resistance (low conductance) then the cell will take a long time to change. if the capacitance is high it will also take a long time to change.

The equation also shows us that the membrane cannot change instantaneously - it changes on the scale of RC.

The equivalent schematic of the cell with only the Potassium channel is:

## Opening a Channel

The opening of the ion channels in a specific order has the following effect.

- Membrane potential starting at -55mV
- Open a Sodium channel
- Membrane voltage moves toward the Sodium reversal potential (+60 mV)
- Then a Chloride channel opens and ...
- the membrane potential starts moving toward the Chloride reversal potential (-70 mV)
- When a Potassium channel opens ...
- the membrane potential moves toward the Potassium reversal potential -90 mV

This is the basis of ion channel dynamics.

# **Next: ***The Simulator*

*The Simulator*